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Image Search Results
Journal: bioRxiv
Article Title: BEVA2.0: Modular Assembly of Golden Gate-Compatible Vectors with Expanded Utility for Genetic Engineering
doi: 10.1101/2024.12.04.626608
Figure Lengend Snippet: Dashed vector backbones reflect modules from BEVA1.0 . Solid vector backbones reflect BEVA2.0 expansion modules presented in this work. Colored rounded rectangles reflect parts, colored by module type. Colored circles reflect sticky overhangs generated by BsmB1 and ligated by DNA ligase in Golden Gate cloning reactions.
Article Snippet: Golden Gate cloning reactions were performed using forty fmol of plasmid parts in a reaction mix recipe of 1 µl restriction enzyme (BsmBI-v2, Esp3I, or BsaI-HF-v2) (New England Biolabs), 1.5 µl bovine serum albumin, 1 µl
Techniques: Plasmid Preparation, Generated, Clone Assay